Currently, Flow serves as chair of the Winston-Salem Open ATP Tournament, chair of the board of directors of the Winston-Salem Alliance, Inc., chair of the Golden Leaf Foundation, chair of the Wake Forest Baptist Medical Center board of trustees and a member of the board of Atrium Health, and vice chair of the Piedmont Triad Partnership. He has been actively involved in the repurposing of nearly 4 million square feet of Reynolds manufacturing properties into today’s Innovation Quarter through roles on the Board of Wake Forest University, Wake Forest Baptist Medical Center, and the Whitaker Park Development Authority, among others. Hear our community share their perspectives in our new video!Īs Chairman and CEO of Flow Companies, with 51 dealership locations throughout North Carolina and Virgina, Don Flow has become a pre-eminent job creator. With a population of 246,000, Winston-Salem has consistently built resources in the arts, medicine, healthcare research, education and commerce. Mark Owens also provided updates on Greater Winston-Salem, Inc’s initiatives in advocacy, small business support, talent and workforce, and economic development, driving momentum for Forsyth County. Wake Forest’s hometown, Winston-Salem, North Carolina, is a culturally rich mid-size city in the Piedmont Triad area of one of the nation’s most progressive states. Since 2018, our entrepreneurial ecosystem has fostered the growth of more than 60 startups – with over 180 million in capital raised.We are outpacing our previous ten-year trends in just the past three years. (AP) Brock Wilken hit three of Wake Forest’s record-tying nine home runs, and the Demon Deacons routed Alabama 22-5 on Sunday to win the Winston-Salem Super Regional. In the ten years prior – 2010 to 2020: 14 projects, 2,604 jobs, and $906 million capital investment. Since 2020: 25 projects announced, 1,615 jobs created, $617 million capital investment.Over 2.4 million impressions in peer cities and major metros so far in 2023 to raise awareness and attract talent.As the Chamber of Commerce for Forsyth County, we are engaging the business community with more than 45 events held so far this year with 2,000+ attendees.It has helped to create or retain more than 40 jobs. Our MBE Grant program – which is open for this year’s round of applications now – has distributed more than $670,000 dollars to 47 Black and Latino Owned small businesses.37 students have received our new Work Ready Credential launched this Spring. ASPIRE has served 150 students to-date and over 30 local companies have participated in hosting paid interns. fertilizer plant is still burning Thursday morning, crews said in a 5:15 a.m. board and Mark Owens, President and CEO, provided an update on Winston-Salem’s economic growth, future opportunities, and progress towards the 2030 Vision: to be the top mid-sized city in the Southeast, to be a more equitable community, and to be the best place to raise a family.īenchmarks of progress towards those goals include: The massive fire that started Monday night at the chemical-filled Winston Weaver Co. We will always strive to offer the highest standard of customer service always, without hesitation, for all residents and visitors of the County of Wake.Cathy Pace, Chair of the Greater Winston-Salem, Inc.
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However, all patrons are encouraged to: Call the Zoo first 40. The Zoo is allowing walk-up admissions for patrons using the pass voucher.The Pass voucher is redeemable for four weeks/28 days from the date it was issued.The Pass voucher is redeemable for three (3) free admissions.The Pass voucher is redeemable Monday through Friday excluding blackout dates.Limit one check out per year, per household. Present your pass voucher and your library card at the Zoo Atlanta front gate for admission. Please be sure to save your pass voucher as it is required for Zoo admission. When you return the DVD to the Ask Us Desk, you will receive a Library Pass voucher. To redeem the Library Pass voucher, check out the Zoo Atlanta Animal Explorers: History of Zoo Atlanta DVD. Lost passes incur a $60 replacement fee.Ĩ00 Cherokee Avenue SE, Atlanta GA 30315.The Family Theatre Pass must be presented at the Shakespeare Tavern® Playhouse Box Office to receive your free tickets.0) or email your requested show and date to reserve your free tickets. Visit to select your show, date, and time. Check out the Pass from your local library.To reserve free tickets using the Pass, patrons must:.Once you have worked at the Center for Puppetry Arts, you’ll be part of a network of Atlanta artists and theaters, making it easy to. The Center for Puppetry Arts is a unique cultural treasure - a magical place where children and adults are educated, enlightened and entertained. Tickets are available on first-come, first-served basis and may not be available for closing weekend performances or non-Atlanta Shakespeare Company productions, such as companies renting the Playhouse. As an employee of the Center you will be able to receive discounted admission to shows and events for friends and loved ones, as well as standard compensation. Specialties: Touching lives through the art of puppetry.Visitors can create a Rudolph the Red-Nosed Reindeer Shadow Puppet. The puppet is based on the style of puppetry or character used in the production. Your All-Inclusive Performance ticket includes the show, admission to the Worlds of Puppetry Museum, and the Create-A-Puppet Workshop. All tickets redeemed using the Family Theatre Pass are for general admission only and may not be used for special events or food or beverages. Rudolph the Red-Nosed Reindeer Shadow Puppet.Tickets available through the Pass are only available within the fourteen (14) day circulation period, so you may only select a performance during those fourteen (14) days.The Pass has a circulation period of seven (7) days including one (1) renewal, for a total checkout period of up to fourteen (14) days. A wonderland for visitors of all ages and hands down one of Atlantas most unique attractions, the museum expanded in 2015 houses.The Family Theatre Pass allows for up to four (4) free tickets to a performance at the Shakespeare Tavern® Playhouse or it can be used for four (4) free box seat tickets to a tiered seating performance.SHAKESPEARE TAVERN® PLAYHOUSE FAMILY THEATREĤ99 Peachtree Street NE, Atlanta, GA 30308 | Join the game and collect challenges and quests while rewarding yourself. To help gamers get more comfortable in the game, Avakin Life also rewards gamers with valuable prizes every day. Explore the huge online world where you will have the opportunity to go bar-hopping, swimming or hang out on the beach and much more. Become a famous model, skilled worker and anything you want by following all the features. In addition, you have the option to find the ideal job you always wanted. Switch between different looks by different outfits or simply by changing your hairstyle. 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Avakin Life MOD APK is a 3D virtual game for young people that can be described as a virtual world playground, published by Lockwood Publishing Ltd. “How do you look 6,7 but are 5,3,” Joss commented. In addition to stunning people with her long torso, Emmie shocked people with her petite stature which she claims is just 5’2”, a number she later changed in a video stating she is 5’3”. 5.4M views 2 years ago TikTok FullBodyWorkout WithMe. “Why are you not a model□□□□,” said Nicole Callan. Commenters compared this TikToker to the Annelids (pictured) from Men in Black. Explore the latest videos from hashtags: tiktokbodydance, tiktokdancebody, tiktokbody, bikinibodydance, tiktokbodydance, bodybodydance, tiktokbody, bodydancetiktok, bikinibodytiktokdance, tiktokhalfbodydance. Something New Remix DC - G-Force Official. Emmie modeled her frame in a TikTok video last month. TikTok video from Henrick Epa (erickgids19): 'Hi Every body, Just my body. “Gorgeous little centipede □,” said Charlieann on another one of her videos. “but her figure is perfect and I don’t see a problem whys everyone so hateful and jealous □,” Sid Annoreilly22. “Every body is different and nothing wrong with that (you own it girl !! Respect) ❤️,” Shan_w2817 said. Other TikTokers rushed to Emmie’s defense and praised her unique build. Mine is non existent,” said Shey the Great. “When’s the next Men In Black movie coming ,” wrote Taylor Mawn on Emmie’s video. TikToker Austin Brown, who goes by posted a response to one of Emma’s dance videos along with a picture of Neeble, one of the alien worms from Men in Black.Ĭommenters trolled her both on his stich and on Emmie’s videos on her TikTok. Why not Jumping on the trend of my own song lol touchmybody, Carey wrote under her video. Why not Jumping on the trend of my own song lol touchmybody. Some commenters were complimentary, but others compared her body shape to a species of alien worms from the 1997 Sci-Fi comedy film Men in Black. Mariah Carey is enjoying TikTok’s viral Touch My Body dance and the singer is getting in on the fun with her twins. I explain 2 different ways to learn this dance move, so if the body roll. Some TikTokers are going to great lengths to defend a fellow social media user with a long torso after trolls compared her to an alien.Įmmie, a 22-year-old who goes by on TikTok, posted a video of herself dancing last month which has since garnered over a million views and scores of comments. The BODY ROLL is used in A LOT of TikTok dances So here's an EASY step by step tutorial on how to do the body roll. I went to an all-you-can-eat buffet - I was asked if I was pregnantįestival-goer claps back at ‘revolting’ male body-shamers: ‘Fat people exist!’ Mariah Carey Touch My Body Challenge Dance Compilation dance challenge Best TikTok, Dubsmash and Instagram Mariah Carey Touch My Body Challenge Compilation. Gwyneth Paltrow’s ‘fat’ double from ‘Shallow Hal’: I almost starved to death after role (Since the song released two weeks ago, “WAP” has been used as the audio for just under 200,000 videos on the app, while the hashtag #WAPdance has amassed over 2.7 million views.I’m a ‘fat girl’ - here are my ‘life saving’ hygiene tips for plus-size people Other artists have acknowledged the power of the TikTok dance as well Doja Cat included Haley Sharpe, the TikTok user who created the viral choreography to her song “Say So,” in the official music video for the track, while Cardi B has vowed to learn the choreography to the dance challenge that choreographer Brian Esperon debuted on TikTok to her song “WAP” with Megan Thee Stallion. This dolphin dance move is used in A LOT of TikTok dances, so thats why I made this detaile. The dance challenge began before the song had even dropped. EASY Dolphin TikTok tutorial where I break it down step by step. For Drake, no stranger to the potential of a viral challenge, having the “Toosie Slide” hit #1 on the Billboard Top 100 appeared to be a clever feat of reverse engineering, thanks to his instructive lyrics that were perfect for a soundbite, and the “leak” of a hip hop dancer-slash-influencer performing choreography to it. But the power of a trending dance extends beyond the views of a viral video this year so far, TikTok dance challenges were responsible for securing top chart spots for artists like Drake, Doja Cat and Megan Thee Stallion, whose songs provided the addictive soundtracks for these dance trends. In questo periodo Frankenthaler lavora sul concetto di “fare di più per ogni immagine,” per creare opere allo stesso tempo più complesse e complete. Le tele di grande formato Tunis II e Dream Walk Red (entrambe del 1978), esprimono un forte senso di calore, con densi strati di rosso scuro, rosa, cremisi, terra di Siena e rosso scarlatto. In entrambi questi dipinti, i colori caldi della terra sono in contrasto con il blu-verde acqueo, evocando l’incontro tra terra e acqua. In Jupiter (1976) e Reflection (1977), le pennellate sono dense e verticali, e sembrano essere sul punto di dissolversi. Una delle prime tele di quegli anni, Ocean Drive West # 1 (1974), si riferisce esplicitamente all’oceano con le sue bande orizzontali fluttuanti, che sembrano arretrare di fronte a una distesa blu trasparente. La mostra, intitolata Sea Change, comprende undici tele dipinte tra il 1974 e il 1983 fortemente influenzate dagli ampi panorami e dal movimento delle maree di questi nuovi paesaggi. Nell’estate del 1974 Frankenthaler affitta una casa a Shippan Point vicino a Stamford, nel Connecticut, affacciata sulle acque del Long Island Sound, segnando l’inizio di un importante periodo di cambiamento nel suo lavoro. Con la sua invenzione della tecnica soak-stain, ha ampliato le possibilità della pittura astratta, rifacendosi a volte anche al figurativo e al paesaggio in modo unico. Figura di punta della seconda generazione di pittori astratti americani del Dopoguerra, è famosa per il suo ruolo fondamentale nella transizione dall’Espressionismo Astratto al movimento Color Field. Gagosian è lieta di presentare a Roma una mostra di dipinti di Helen Frankenthaler, in concomitanza con l’esposizione dell’artista al Museo di Palazzo Grimani durante la Biennale di Venezia 2019.įrankenthaler è da tempo riconosciuta come una delle più grandi artiste americane del Ventesimo secolo. Pittura/Panorama: Paintings by Helen Frankenthaler, 1952–1992 will be on view at Palazzo Grimani, Venice, until November 17.ĭisegna sull’intera superficie ed al suo interno, colorane alcune parti e trasformala in una specie di mare. The exhibition will be accompanied by a fully illustrated catalogue with an essay by Elderfield. It was organized by John Elderfield, a senior curator at Gagosian. Helen Frankenthaler: Sea Change is the fifth exhibition of Frankenthaler’s work presented by Gagosian since 2013. Its solid green ground is at once the opposite and the outcome of what had begun with the ocean blue monochrome of Ocean Drive West #1. A “kind of sea” was back on land. This, the latest canvas in the exhibition, makes clear that Frankenthaler is no longer thinking of the water and is instead imagining moving over a flat, resistant surface. With Tumbleweed (1982), Frankenthaler sets this painterly incident onto a solid, mid-toned field of green, which turns it away from the allusion of atmosphere-air and water-onto firmer ground, as acknowledged by the title, and the verdant surface. The dabs, dots, and dashes of yellow pigment in Omen presaged the ambitiously scaled horizontal canvases that followed, with thick clumps and trails of darker-colored pigment on a lighter atmospheric ground in Sacrifice Decision (1981), or lighter on darker in Eastern Light (1982). In Feather (1979), Omen (1980), and Shippan Point: Twilight (1980), colors mix, layer, and fold into each other to produce soft, unnameable hues. In this period, Frankenthaler talked about “doing more to each picture,” to create something at once more complex and complete. The large, wide canvases, Tunis II and Dream Walk Red (both 1978), emanate warmth, with densely layered dark red, rose, crimson, sienna, and scarlet. In both of these paintings, the warm earth colors of the bands contrast with a cool, aqueous blue-green, evoking the meeting of land and water. In Jupiter (1976) and Reflection (1977), the bands are clustered together and turned to the vertical, appearing to all but dissolve. One of the earliest canvases, Ocean Drive West #1 (1974), is explicitly oceanic with its floating horizontal bands, seeming to recede across an expanse of transparent blue. Sea Change comprises eleven canvases that Frankenthaler painted between 19, which reflect her responses to the changing appearance of the wide vistas and moving tides. In the summer of 1974, Frankenthaler rented a house at Shippan Point in Stamford, Connecticut, facing the waters of Long Island Sound, marking the beginning of an important period of change for her work. Gagosian is pleased to present an exhibition of paintings by Helen Frankenthaler in Rome, coinciding with an exhibition of her work at the Museo di Palazzo Grimani, Venice, on the occasion of the 58th Venice Biennale. Draw in and on the entire surface of it, color it in part, and make it a kind of sea. Scrabble was created by American architect Alfred Mosher Butts in 1938. 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Satellite communications typically involve a VHF uplink in the 2 meter band, and the azimuth This Delta Loop is a three-sided antenna suspended high in the air by vertical supports, such as tall evergreen trees. Trying To Make Homemade Antenna For Channel 10 Tv Fool. Several factors must be considered in the design of an RDF loop. Here is a 70cm (440 Mhz) J-Pole antenna that is inexpensive, and easy to build. It will not compete with a good high-gain directional antenna, but it sure beats rabbit ears. This preamplifier features a internal … A quick and easy to build loop antenna for shortwave listeining can tune from 5 to 18 Mhz Dave's Loop Antennas N2DS home made loop page collection. It would also be useful to have a broadband receiver covering 100KHz to 1GHz, for example the ICOM T90A or Yaesu VX-2. Some words and phrases indicate specific time frames, and therefore signal the use of the preterite.Īyer (yesterday) anteayer (the day before yesterday) anoche (last night) desde el primer momento (from the first moment) durante dos siglos (for two centuries) el otro día (the other day) en ese momento (at that moment) entonces (then) esta mañana (this morning) esta tarde (this afternoon) la semana pasada (last week) el mes pasado (last month) el año pasado (last year) hace dos días, años (two days, years ago) ayer por la mañana (yesterday morning) ayer por la tarde (yesterday afternoon) There are only three irregular verbs in the imperfect: ser Note: This is not a typo ser and ir do have identical conjugations in the preterite! Ser, ir, dar and hacer are irregular in the preterite: ser To describe the characteristics of people, things or conditions.For actions that “set the stage” for another past action.For actions that were repeated habitually.The imperfect is used in the following situations: To state the beginning or the end of an action.For actions that were part of a chain of events.For actions that occurred during a specific period of time.For actions that were repeated a specific number of times.For actions that can be viewed as single events.The preterite is used in the following situations: Generally speaking, the preterite is used for actions in the past that are seen as completed, while the imperfect tense is used for past actions that did not have a definite beginning or a definite end.Īnother way to view this is that the preterite tells us specifically when an action took place, while the imperfect tells us in general when an action took place. Here are all three regular imperfect verb forms together: hablar Note: The nosotros forms for -ar and -ir verbs are the same in both preterite and present tenses: hablamos, vivimos. Here are all three regular preterite verb forms together: hablar Subjunctive VIII: Actions not yet completed Subjunctive III: Verbs that change orthographically Subjunctive II: Conjugating regular and stem-changing verbs ¿Quién las lámparas de la oficina? (componer) Gabriel García Marquéz un escritor colombiano muy famoso. Tú dos párrafos del texto para entender mejor. ¿Ustedes a sus padres sobre la reunión de hoy? (decir)ġ3. Ellos a la biblioteca después de su entrenamiento. Nosotros en la casa de Pedro desde el viernes pasado. Sara ensalada de frutas para los estudiantes esta mañana. ¿Qué tú el fin de semana pasado? (hacer)ĩ. Niños ¿dónde ustedes los juguetes que compramos ayer? (poner)Ĩ. Luis y su novia ir al concierto el sábado. ¿Qué tú que comprar para tu viaje a Sudamérica? (tener)Ħ. ¿Tus padres a Australia el año pasado? (ir)ĥ. Yo 100 dólares por mis botas de invierno. Yo la dirección del restaurante en Google Maps. Prices as low as just $11 per hour for your first purchase of lessons!ĬLICK FOR YOUR FREE TRIAL CLASS! Interactive Grammar ExerciseĬomplete the following sentences with the Preterite of the irregular verbs given in brackets.ġ.
Anything over 60% has a strong likelihood of cross-reacting, but will need to be verified in an assay and sample specific manner.ĭoes my antibody cross-react with other species? What is the “right number” for homology to predict cross-reactivity?īased on our extensive experience in antibody development and working with thousands of customers, the magic number for almost guaranteed cross-reactivity is 75% homology with the immunogen sequence. 95% of Proteintech's antibodies are raised against recombinant, long-length human proteins, meaning that they have an increased chance of cross-reactivity in non-human models such as mouse, rat, drosophila and zebrafish. Conversely, if you are looking for an antibody to work in other species, use a polyclonal antibody against a recombinant, long-length protein. Therefore if you suspect your antibody of cross-reacting negatively, consider switching to a monoclonal antibody (a homologous IgG antibody population only recognizing one epitope). Remember that polyclonal antibodies have a higher chance or cross-reactivity due to the fact that they recognise multiple epitopes along the immunogen sequence. Cross-reactivity is not always a negative quality, for example cross-reactivity of an antibody for a target across species allows the same antibody to be used in multiple model organisms. Here, you can check for an antibody's potential cross reactivity against that of similar proteins or in other species. This can be achieved by pair-wise sequence alignment using NCBI-BLAST. Thus, testing an antibody for cross-reactivity with closely related proteins is a to ensure accurate results.Ī quick and easy check for antibody cross reactivity is to assess the percentage homology of the antibody immunogen to that of other similar proteins. This is often the case when two antigens have similar structural regions that the antibody recognizes.Ĭross-reactivity can invalidate the results of an experiment and has negative impacts on scientific reproducibility. Cross-reactivity between antigens occurs when an antibody raised against one specific antigen has a competing high affinity toward a different antigen, therefore the antibody is able to recognize a protein which is different to the one it was raised against. So overall, in general terms, antibody specificity helps us discuss if the antibody that we are using in our research (whether it’s a single antibody from a B cell or a pool of antibodies from serum) is able to detect our target protein specifically without cross-reacting with non-specific proteins.An antibody has a specific amino acid sequence (the Fab region) that dictates its affinity for a specific antigen. Techniques for increasing specificity will be discussed on the Monoclonal Antibody Alternative page. These two antibody production techniques will be discussed on the Polyclonal vs Monoclonal Antibodies page.Īlso, because serum contains antibodies against so many antigens (not just the protein that may have been used for immunizations), specificity is typically very low with raw serum. This brings up the issue of how antibodies are typically used, since they can be collected directly from the serum or by isolating the B cell(s) that is producing the antibody of interest. So, one antibody could potentially recognize two or more proteins if these proteins are highly homologous and contain the same epitope.Īlso, it is important to recognize that multiple antibodies will be generated against a typical protein antigen and so any one of these antibodies could potentially cross-react with another protein that contains the same epitope(s). However, it is important to recognize that a particular epitope could potentially appear on more than one protein antigen. This specificity allows precise detection of a target antigen such as a protein while avoiding detection of unrelated proteins that are not of interest. Each individual antibody protein is capable of binding specifically with one unique epitope thanks to the unique Antigen Binding Site located at the tip of the variable region on the antibody. |
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